College of Veterinary Science, Assam Agricultural University, Guwahati-781022, India
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Table 1. Primers used for determination of Salmonella
enterica serotypes by multiplex PCR assay
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Multiplex group |
NCBI Accession No. |
Primer Name |
Primer sequence (5/ - 3/) |
Amplicon size (+ 0.5 bp) |
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All |
AE006468 |
STM1608F |
GTCGGCGAGCTTCACC |
63 |
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STM1608R |
CATCGTAGCAAAGCGCAGTT |
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M-1 |
AE006468 |
STM4525F |
CTGAGCATCCGCTTCATCGC |
239 |
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STM4525R |
ACACTGACCAGCACCAGG |
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M-1 |
AE006468 |
STM2150F |
AGCCTGCATAATCGCAAAGG |
198 |
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STM2150R |
CATCAGCGACACGATAGTGAGA |
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M-1 |
AL627266 |
STY0311F |
TGGGTTGCCACCTATACGTCT |
172 |
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STY0311R |
GTTCACTGGCGTATCCGGTA |
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M-1 |
AE006468 |
STM3845F |
GTGTTTGAAGATGATATAGCCAGT |
138 |
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STM3845R |
TTCCGCTGAAGCAACAATCAG |
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M-1 |
AL627273 |
STY2296F |
CTCTGTTGCAAACCAGATGTTA |
86 |
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STY2296R |
GATGGAGACGATAAGTTTACCAGTAT |
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M-2 |
AF370716 |
SdflF |
GGCGATATAAGTACGACCATCATGG |
225 |
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SdfIR |
GCACGCGGCACAGTTAAAA |
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M-2 |
AE006468 |
STM0171F |
GACCCCGGATTTTTTGAGAA |
179 |
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STM0171R |
ACCACGGAGAGACAGTTCAGAT |
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M-2 |
AE006468 |
STM0716F |
CCGGAAAGAAACCGCTGCTTA |
125 |
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STM0716R |
TTGAGGCGCCGGATATAAAG |
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M-2 |
AL627273 |
STY2349F |
GGCGAGTTCTACGCAGAGATAA |
91 |
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STY2349R |
CCAGCGAAATGTCACAGTGA |
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M-3 |
AE006468 |
STM2771F |
CCATTGGATGTCCTCACACC |
231 |
|
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STM2771R |
GGCAATTCTTGAAGAATTATCAGG |
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M-3 |
AE006468 |
STM4538F |
AGCGGAATGATGGAAAGCCTA |
118 |
|
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STM4538R |
CTTCTCCCGTACCAAACGTA |
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M-3 |
AE006468 |
STM0839F |
AAGGTTTCCCGCTGACCTCT |
81 |
|
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STM0839R |
AGGTACGCATCCATACGCCT |
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M-4 |
AE006468 |
STM3518F |
CGCGGAAGCGGTAGCTA |
230 |
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STM3518R |
CAAGATAGCCACTTTCGGTTG |
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M-4 |
AL627266 |
STY0345F |
GGTATGTCGTTCAAACAGGAAT |
152 |
|
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STY0345R |
CACTGCCGAGCAGTATGAG |
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M-4 |
AE006468 |
STM1350F |
GGAACACATCTTGCCAGGT |
108 |
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STM1350R |
CCGGCACAGAAGGAATGC |
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