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College of Veterinary Science, Assam Agricultural University, Guwahati-781022, India
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Table 1. Primers used for determination of Salmonella enterica serotypes by multiplex PCR assay

Multiplex group

NCBI Accession No.

Primer Name

Primer sequence (5/ - 3/)

Amplicon size

(+ 0.5 bp)

All

AE006468

STM1608F

GTCGGCGAGCTTCACC

63

 

 

STM1608R

CATCGTAGCAAAGCGCAGTT

 

 

M-1

AE006468

STM4525F

CTGAGCATCCGCTTCATCGC

239

 

 

STM4525R

ACACTGACCAGCACCAGG

 

M-1

AE006468

STM2150F

AGCCTGCATAATCGCAAAGG

198

 

 

STM2150R

CATCAGCGACACGATAGTGAGA

 

M-1

AL627266

STY0311F

TGGGTTGCCACCTATACGTCT

172

 

 

STY0311R

GTTCACTGGCGTATCCGGTA

 

M-1

AE006468

STM3845F

GTGTTTGAAGATGATATAGCCAGT

138

 

 

STM3845R

TTCCGCTGAAGCAACAATCAG

 

M-1

AL627273

STY2296F

CTCTGTTGCAAACCAGATGTTA

86

 

 

STY2296R

GATGGAGACGATAAGTTTACCAGTAT

 

 

M-2

AF370716

SdflF

GGCGATATAAGTACGACCATCATGG

225

 

 

SdfIR

GCACGCGGCACAGTTAAAA

 

M-2

AE006468

STM0171F

GACCCCGGATTTTTTGAGAA

179

 

 

STM0171R

ACCACGGAGAGACAGTTCAGAT

 

M-2

AE006468

STM0716F

CCGGAAAGAAACCGCTGCTTA

125

 

 

STM0716R

TTGAGGCGCCGGATATAAAG

 

M-2

AL627273

STY2349F

GGCGAGTTCTACGCAGAGATAA

91

 

 

STY2349R

CCAGCGAAATGTCACAGTGA

 

 

M-3

AE006468

STM2771F

CCATTGGATGTCCTCACACC

231

 

 

STM2771R

GGCAATTCTTGAAGAATTATCAGG

 

M-3

AE006468

STM4538F

AGCGGAATGATGGAAAGCCTA

118

 

 

STM4538R

CTTCTCCCGTACCAAACGTA

 

M-3

AE006468

STM0839F

AAGGTTTCCCGCTGACCTCT

81

 

 

STM0839R

AGGTACGCATCCATACGCCT

 

 

M-4

AE006468

STM3518F

CGCGGAAGCGGTAGCTA

230

 

 

STM3518R

CAAGATAGCCACTTTCGGTTG

 

M-4

AL627266

STY0345F

GGTATGTCGTTCAAACAGGAAT

152

 

 

STY0345R

CACTGCCGAGCAGTATGAG

 

M-4

AE006468

STM1350F

GGAACACATCTTGCCAGGT

108

 

 

STM1350R

CCGGCACAGAAGGAATGC